Analysis of secondary metabolites, antioxidant properties, HPLC analysis, and protein content of Calostoma insigne in Cambodia
DOI:
https://doi.org/10.62992/ynsqeq88Keywords:
Antioxidant, HPLC, Mushroom, Protein, Secondary metabolitesAbstract
Background: Calostoma insigne (C. insigne) is a wild edible mushroom commonly found in the forests of Southeast Asia. In Cambodia, it is traditionally consumed as a dessert, with locals believing in its health benefits. However, there is a significant lack of research investigating its therapeutic properties and chemical compounds, particularly within the Cambodian context.
Objectives: This study aimed to explore the secondary metabolites, protein content and antioxidant activities in C. insigne.
Methods: Methanolic extract of C. insigne was utilized to define the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities was screened using ferric reducing antioxidation power assay (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging in microplate spectrophotometers. Protein content was performed using Kjeldahl method. HPLC detected gallic acid, chlorogenic acid, and luteolin in the crude extraction.
Results: The analysis showed significant levels of secondary metabolites with TPC (9.28 ± 0.51 mg GAE/g of CE), TFC (242.41 ± 23.82 mg quercetin equivalent/g of crude extract). Antioxidant activities of DPPH radical revealed a concentration dependent manner (P < 0.05), and HPLC detected luteolin (15.83 ± 0.01 mg/g per crude extraction). TFC showed a strong statistically significantly negative correlation with FRAP value explained r = -0.879, P < 0.05. TFC could be the biomarker in extraction with high-reducing antioxidant power.
Conclusion: This study confirmed the presence of secondary metabolites, their potential as antioxidant activities, and protein content for further development in food products, pharmaceutical and natural health products.
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